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High Performance Liquid Chromatography

High performance liquid chromatography (HPLC) is an analytical chromatographic technique used to separate ions or molecules dissolved in a solvent. Unlike gas chromatography, the mobile phase is a liquid which is forced under high pressure through a column packed with a sorbent. Each substance is separated from the others by passage through the column, which produces a separate peak in the detector output. Various separation methods, including adsorption, gel filtration, ion-exchange, and partition, are used.

HPLC Septa Characteristics

Liquid Chromatography (LC), HPLC, and LC-MS require a slightly different septa design. Because the sample is less volatile, straight PTFE septa or pre-slit Silicone/PTFE septa designs may be used. A straight PTFE septum (preferably bonded into the cap) offers excellent solvent resistance and chemical compatibility, but does not re-seal upon puncturing. Pre-slit Silicone/PTFE septa prevent needle coring, offer moderate re-sealing, and provide for excellent sample-draw reproducibility. Use of non-pigment silicones and PTFEs reduce the potential for contamination.

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